Dot Blot 96
The Dot Blot 96 system provides easy and reproducible methods for immobilising, concentrating and binding proteins, DNA or RNA in solution onto membranes. Typical applications are Dot Blot hybridisation of plasmid DNA, small RNA and DNA fragments, cloned bacteria, lymphomas and viral nucleic acids, screening of recombinant clones, screening of cell surface antigens, as well as filtration and immobilisation of small volumes onto immobilising matrices.
Easy to use
The Dot Blot 96 works without movable O-rings, making it extremely easy to set up. Its innovative sealing system eliminates lateral leakage that can cause cross-contamination between wells. Each well is numbered and lettered compatible to the standard 96-well plate format. Multichannel pipets for rapid handling of samples can be used.
Up to 96 samples can be loaded in short time. The maximum sample volume is 350 µl per well.
Dot Blot 96 features a buffer tank design that eliminates the need for trapping bottles (separators), facilitates buffer recycling, and protects any vacuum pump from buffer aspiration. The use of model MP86 vacuum pump (included in the Dot Blot 96 System) is recommended as this pump offers controlled and adjustable vacuum to prevent damage of the transfer membrane.